Comparative Effects of Platelet-Rich Plasma and Erythropoietin on Oxidant/Antioxidant Balance in Diabetic Rats

Diabetes is a persistent metabolic disease represented by hyperglycemia that leads to oxidative stress caused by oxidant/antioxidant imbalance. Platelet-Rich Plasma (PRP) has been utilized clinically to stimulate tissue repair and cell proliferation in various medical fields. Erythropoietin (EPO) has shown protective effects in various tissues, mitigating ischemia-reperfusion injury and promoting tissue regeneration. This study aimed to assess the effects of PRP and EPO on the oxidant/antioxidant balance in diabetic rats. A total of ۳۰ male rats were assigned into five groups: ۱. Control; ۲. Diabetic control, diabetes induced using streptozotocin (STZ); ۳. Diabetic + PRP: PRP was administered subcutaneously at ۰.۵ ml/kg two times a week for four weeks in diabetic rats; ۴. Diabetic + EPO: EPO was administered at ۳۰۰ units/kg three times a week for four weeks in diabetic rats; and ۵. Diabetic + PRP + EPO: a combination of both PRP and EPO was administered for four weeks. Diabetic rats showed significant reductions in superoxide dismutase (SOD), glutathione peroxidase (GPX), and glutathion (GSH) levels and a rise in malondialdehyde (MDA) concentration in contrast to controls (p<۰.۰۵). PRP and EPO treatments significantly increased SOD, GPX, and GSH quantities (p<۰.۰۵) and lowered MDA concentrations compared to untreated diabetic rats. The combination therapy group exhibited the highest improvements in antioxidant activities. This study demonstrates that both PRP and EPO exhibit significant antioxidant effects in diabetic rats, with the combined treatment showing the most pronounced improvements in oxidative stress markers. These results provide a foundation for potential clinical applications of PRP and EPO in enhancing antioxidant defenses and reducing oxidative damage in diabetic patients.

کلیدواژه ها:

diabetes ، oxidative stress ، platelet-rich plasma ، Erythropoietin ، Antioxidant enzymes