Bisubstrate Kinetic Model for Enzymatic Decolorization of Reactive Black ۵ by Coprinus cinereus Peroxidase

In this study, decolorization of the diazo dye, Reactive Black ۵ (RB۵) in a Coprinus cinereus peroxidase-catalyzed reaction has been investigated. A bisubstate kinetic model for the reaction rate based on the Ping-Pong mechanism was assumed for the enzymatic decolorization. Experimentswere conducted at different RB۵ and hydrogen peroxideconcentrations in a batch mannerto estimate the intrinsic kinetic parameters. These parameters were used for the modeling of decolorization in a continuous reactor that was compared with experimental results. An acceptable agreement was observed between the model and experimental data.

کلیدواژه ها:

Coprinus cinereus peroxidase ، Decolorization ، Reactive black ۵ ، Enzymatic reaction ، Ping-Pong mechanism