Soybean Oil Degumming by Immobilized Phospholipase A۱

In the present study, we investigated the ability of an immobilized phospholipase A۱ (PLA۱) in degumming of soybean oil. The enzyme was immobilized by simple adsorption on bentonite without any further modification. The free and immobilized PLA۱ were characterized by Fourier Transform InfraRed (FT-IR) spectroscopy and X-Ray Diffraction (XRD). The immobilization of PLA۱ lowered the Energy of Activation (EA) from ۱۵۵.۶۴ to ۲۷.۱۳ kJ/mol, resulting in higher catalytic efficiency of PLA۱. Thermal stability of the immobilized enzyme was found to be higher compared to free PLA۱. Moreover, under the condition of pH ۵.۵ and T = ۵۰ °C, the phosphorus content was reduced to less than ۱۰ ppm after ۴-۵ h for free PLA۱ and after ۷ h for immobilized PLA۱.

کلیدواژه ها:

Enzymatic degumming ، Immobilization ، Bentonite ، Phospholipase ، Soybean oil