A Comparative Study between Different Tunisian Propolis Essential Oils and Their Antioxidant Activities

Propolis is a resinous substance collected by bees from plants with a complex and variable chemical composition. Propolis and its fractions possess multiple biological activities. This study focused on a chemical and statistical comparison between four Tunisian Propolis Essential Oils (PEO) and its antioxidant activities. Volatile oils were extracted by hydrodistillation and analyzed by GC-MS. Essential oil yield varied from ۰.۰۹۵% to ۰.۳۲۴%. A total of ۵۹ volatile components were identified mainly dominated by sesquiterpenes and diterpenes hydrocarbons. Six major components were found in all samples collected from the four different locations α-Cedrol, Manoyl Oxide, Manool, Totarol, Tricosane, and Eicosane. The antioxidant activities of Tunisian propolis essential oils have been evaluated using two methods: β-carotene-linoleic acid bleaching and DPPH radical scavenging assays and the results were compared with the standard antioxidant (Triolox). PEO from Bizerte region presented a lower IC۵۰ value (۳۰.۵ mg/mL) than that of the standard antioxidant Trolox (IC۵۰ = ۴۰.۰۵ mg/mL) indicating high antioxidant capacity using DPPH assay but for the b-carotene-linoleic acid bleaching assay, PEO from Zouarine region had the lowest value of (IC۵۰ = ۲۶.۵ mg/mL) compared to standard (IC۵۰ = ۳۱.۲۵ mg/mL). Our findings demonstrated that Propolis Essential Oil (PEO) possess high antioxidant activities and may be suggested as a new potential source of natural antioxidant.

کلیدواژه ها:

Tunisian Propolis ، essential oil ، GC/MS ، Antioxidant Activity ، α-Cedrol