Enzymatic Degumming of Soybean Oil by Immobilized Phospholipase A۱ on Plasma Surface Modified Chitosan Nanofibrous Membrane

The paper presents the enzymatic degumming of soybean oil by immobilization of phospholipase A۱ (PLA۱) on atmospheric plasma surface modified chitosan nanofibrous membrane. As evidenced by Scanning Electron Microscopy (SEM) images, chitosan nanofibers exhibited a number of properties indicating this polymer as a good enzyme carrier. Fourier Transform InfraRed (FT-IR) spectroscopy showed the reaction between the enzyme and functional groups of chitosan nanofibers. The properties of immobilized PLA۱ were compared to free PLA۱ in order to evaluate the possibility of using the immobilized enzyme for the degumming of soybean oil. The pH tolerance and thermal stability of the immobilized PLA۱ were significantly improved. In addition, the immobilized PLA۱ can be easily recovered and retained at ۸۰% of its initial activity after ۱۰ times of recycling. The enzymatic degumming process was carried out at ۶۰°C and pH ۶.۰. The residual phosphorus content decreased to ۸.۶ mg/kg after ۶ h, which is to meet oil safety standards and suitable for the physical refining of soybean oil.

کلیدواژه ها:

Enzymatic degumming ، Phospholipase A۱ ، Immobilization ، Soybean oil ، chitosan nanofibers