Synthesis of ۶-(Phenanthrene-۳-yl)-۲,۳-dihydro-۱,۲,۴,-triazine-۳-thione as a New Reagent for the Non-Extractive Spectrophotometric Determination of Cu(II)

A simple, sensitive, and non-extractive spectrophotometric method was developed for the determination of copper (II) using a newly synthesized chromogenic reagent, ۶-(phenanthrene-۳-yl)-۱,۲,۴,-triazine-۳-thione(PhDTT), in Tween ۸۰ neutral surfactant. To synthesize the reagent (PhDDT), amyl nitrite was added to ۳-acethylphenanthrene and refluxed for ۲۶ hours to produce ۲-oxo-۲-(phenanthren-۳-yl) acetaldehyde oxime (I). The reaction of compound (I) with thiosemicarbazide yielded the reagent (PhDDT). Copper (II) forms a red colored complex with ۶-(phenanthrene-۳-yl)-۱,۲,۴,-triazine-۳-tion which shows maximum absorbance at ۴۶۰ nm in the pH range ۹.۰-۱۱.۰. Beer’s law was obeyed over the concentration range of ۱.۴-۱۰ µg/mL Cu۲+ with r۲ = ۰.۹۹۹.  The limit of detection was ۰.۴۸ μg/mL of copper (II). The within-day and between-day precision values were in the range of ۰.۳۸-۳.۳%. The method was tested by analyzing the amalgam samples. The results were in good agreement with the atomic absorption.

کلیدواژه ها:

spectrophotometric method ، Copper determination ، ۶-(phenanthrene-۳-yl)-۱ ، ۲ ، ۴ ، -triazine-۳-thione ، micellar media