Using Chemometrics Methods for Determination of Aripiprazole and Quetiapine as Antipsychotic Drugs in Pharmaceutical Mixture and Biological Fluid by Spectrophotometry Method Based on Continuous Wavelet Transform and Multivariate Calibration

In this study, two multivariate calibration methods, including partial least squares (PLS) and principal component regression (PCR), as well as continuous wavelet transform (CWT) along with spectrophotometry technique were developed for the simultaneous analysis of Aripiprazole (ARI) and Quetiapine (QTP) in the pharmaceutical formulation and biological fluid. The linear range of ARI and QTP was ۱-۳ and ۲-۱۰ μg/mL, respectively for the proposed methods. The root means square error (RMSE) of ARI and QTP related to the test set was obtained ۰.۰۱۴, ۰.۰۷۵۸, and ۰.۱۹۴, ۰.۸۸۲ for PLS and PCR methods, respectively. Also, the mean recovery of ARI and QTP was ۹۹.۹۵, ۱۰۰.۰۴%, and ۹۷.۳۸, ۹۸.۸۳% for PLS and PCR models, respectively. Among various families of wavelets in CWT technique, the Coiflet (Coif۳) and Symlet (Sym۲) families were selected to determine the value of ARI and QTP, respectively. In this method, the Limit of Detection (LOD) and Limit of Quantification (LOQ) values was found ۰.۰۰۳۳, ۰.۰۲۰۰, ۰.۲۷۶۴, ۰.۳۴۸۶ μg/mL for ARI and QTP, respectively. The mean recovery values of ARI and QTP in synthetic mixtures for CWT approach were ۹۶.۹۸%, ۹۸.۹۴%, respectively. A one-way analysis of variance (ANOVA) test was applied to compare the results of both mentioned chemometrics models and High-Performance Liquid Chromatography (HPLC) as a reference method. No significant difference was observed between these methods.

کلیدواژه ها:

Spectrophotometry ، Multivariate calibration ، Continuous Wavelet Transform ، Aripiprazole ، Quetiapine ، Biological fluid