Optimization of Processing Time, Temperature, and Stirring Rate to Synthesize the Ag Nanoparticles Using Oregano Extract

The plant-based extract can be used to synthesize silver nanoparticles (Ag NPs) as a reducing agent. In the present study, Oregano leaves’ extracts were extracted using ethanol to synthesize Ag NPs. The effects of different parameters such as the processing time, temperature, and stirring rate on the mean particle size, concentration, and zeta potential of the synthesized Ag NPs solutions were optimized using Response Surface Methodology (RSM). At the optimum condition, which includes processing time (۳۰.۴۸ min), temperature (۷۰ ºC), and stirring rate (۳۷۰.۵۳۰ RPM), Ag NPs were obtained with ۳۳ nm of the mean particle size, ۷۶.۱۰۹ ppm of concentration, and +۱۷.۲ mV of zeta-potential. In this condition, Ag NPs displayed high antibacterial activity against Gram-negative and Gram-positive bacteria. In addition, the maximum antioxidant activity of ۱۱.۷% was obtained at optimum synthesizing conditions.

کلیدواژه ها:

Green synthesis ، Silver nanoparticles ، Oregano extract ، Response surface methodology (RSM) ، Optimization ، Antibacterial activity