Fabrication and Investigation of ۳D Scaffold Using Hydroxyapatite and Gelatin Nanoparticles for Bone Cancer Treatment with Sufficient Chemical Stability

During the last few decades, with the advancement of the science of knowledge of materials and the ability to investigate their structure in molecular and atomic dimensions, this ability has been created for humans to think of a way to target medicine to the desired points of the body with the help of various tools in nanotechnology. In order to release the drug exactly at the desired point and release it in sufficient quantity, he should conduct many researches and studies in this field. Today, three-dimensional structures are used as suitable drug carriers in various forms to deliver the desired amount of drug to the target points and release it at a controlled rate. In this study, a three-dimensional scaffold based on poly-caprolactone was used. Three different percentages of ۱, ۳ and ۵% by weight of hydroxyapatite (HA) were used in the made inks. Morphology studies and identification of elements showed that ۳D PCL۳%HA scaffold is more suitable than other scaffolds. Based on this, gelatin and HA of ۱, ۳ and ۵% by weight were used to coat the PCL۳%HA scaffold. Studies were done on the final scaffolds. In order to investigate the initial morphology of the ۳D scaffold, an optical microscope was used. Investigation of HA particles was investigated using SEM. EDS test studies showed that with the increase in the weight percentage of HA, the atomic ratio of calcium to phosphorus also increased. The compressive strength of these scaffolds PCL۳%HA-۳%HAG, PCL۳%HA-۱%HAG and PCL۳%HA-۵%HAG has reached ۳۵.۰±۸.۶, ۴۴.۰±۳.۶ and ۴۷.۰±۵.۶, respectively. The results of this study show that despite coating the printed scaffolds with gelatin,  no significant increase in the Young's modulus of the scaffolds was observed. After ۲۴ hours, the PCL۳%HA-۵%HAG scaffold had the highest swelling percentage of ۹۸%. Also, after ۱۲ days, ۳۳% of the scaffold was destroyed. The survival rate of the scaffold is ۱۰۵% and its release rate in ۱۰ hours is ۴۵%. According to the conducted studies, it can be stated that PCL۳%HA-۵%HAG scaffold containing anticancer drug is a suitable candidate for use in bone tissue engineering.

کلیدواژه ها:

Polycaprolactone ، Gelatin ، Hydroxyapatite ، ۳D printing ، Bone cancer