Design of Experiment Methodology for Mannoprotein Industrial Scale Manufacturing Bioprocess: Optimization from a Yeast Cell Factory

Kluyveromyces marxianus is a yeast species with various industrial applications. It's known for its ability to metabolize a wide range of substrates, including lactose, xylose, and cellobiose, which makes it useful in various biotechnological processes. In order to Design of Experiment (DOE) and design an optimized method for extracting and purification of mannoprotein using three homogenization, alkalinity, and bio-emulsifier extraction methods, as well as model data at three levels based on three factors: acidity, temperature, and the mannoprotein extraction method, this study looked at the growth of the yeast Kluyveromyces marxianus (IBRC-M ۳۰۱۱۴) in a ۳۰-L fermentor to determine its optimized yield. Mannoprotein was extracted (in ۲۷ runs with ۳ parts) using QUALITEK-۴ software and the Taguchi method. In the designed stage, various temperatures (۲۵, ۳۰, and ۳۵ °C) and pHs (۲, ۵, and ۷) were used in the homogenization, alkaline, and bio-emulsifier methods. The growth of the yeast K. marxianus in a fermenter (bioreactor) showed that the maximum biomass was obtained from the scale increase inside the reactor. This indicated that the maximum fermentation biomass ۳۴.۰۲ (g/L) was obtained from K. marxianus in ۳۰ h at pH ۴.۶, ۲۹ °C, ۵۰۰ rpm, oxygen ۱۹.۷, inlet air volume ۱ vvm۳, and ۳۶.۶ DO. The maximum amount of mannoprotein ۸.۲۴۳ (mg ۱۰۰ m/L) from ۱۰ (g/L) yeast biomass was extracted by the alkaline method with pH ۵. In the bio-emulsifier method, the mannoprotein extraction was maximized at pH ۷. The homogenization approach fared better than the alkaline method overall in terms of performance. In contrast, the alkaline method outperformed the other two methods homogenization and bioemulsifier in terms of volume or quantity. Mannoproteins play important roles in various biological processes and have several applications in different industries. Mannoproteins have potential applications in drug delivery and as carriers for bioactive compounds due to their biocompatibility and ability to interact with cells and tissues.

کلیدواژه ها:

Mannoprotein ، K. marxianus ، Optimization ، Design Of Experiment (DOE) ، Bioprocess