Media Selection for Poly(hydroxybutyrate) Production from Methanol by Methylobacterium Extorquens DSMZ ۱۳۴۰

Plackett-Burman design was used for selection of important media components such as carbon and nitrogen sources and minerals which affect poly(hydroxybutyrate) production and cell growth of Methylobacterium extorquens DSMZ ۱۳۴۰. Among the studied variables, nitrogen and phosphorus sources, MgSO۴ and most of the trace elements were found to be significant variables for PHB production from methanol. At best condition (based on PHB concentration), dry cell weight, PHB content and PHB concentration were ۳.۸۱ g/L, ۲۱.۲۳ %, and ۰.۸۰۹ g/L, respectively. It was also found that most of the trace elements and phosphorus sources were influential parameters on the growth of microorganism but the kind of nitrogen source was not. The experimental results showed that deficiencies of nitrogen sources (NH۴Cl and NH۴NO۳), phosphorus sources (K۲HPO۴ and Na۲HPO۴) and MgSO۴ in medium, increased PHB accumulation.

کلیدواژه ها:

Methanol ، Poly(hydroxybutyrate) (PHB) ، Methylobacterium extorquens ، Plackett-Burman Design (PBD)