Influence of Different Nitrogen Sources on Amount of Chitosan Production by Aspergillus niger in Solid State Fermentation

In this study the effect of different nitrogen source substrates on the amount of chitosan production by Aspergillus niger was investigated. A. niger PTCC ۵۰۱۲ from the Persian Type Culture Collection (PTCC) was grown on soy bean, corn seed and canola residues at ۳۰ °C for specified cultivation days under sterilized conditions. Chitosan was extracted from the fungal mycelia using hot alkaline and acid treatment. The results were shown that soy bean residue at moisture of ۳۷% and ۸.۴۰.۲۶ % of nitrogen content produced the highest amount of chitosan (۱۷.۰۵۳۰.۹۵ g/kg of dry substrate), after ۱۲ days of incubation. Corn seed residue produced very low amount of chitosan (۱.۹۰.۴ % of nitrogen content). The chitosan was analyzed by FTIR spectroscopy and its spectrum was recorded.

کلیدواژه ها:

Chitosan ، Soybean residue ، Canola residue ، Corn seed residue ، Aspergillus niger ، Fungus ، Solid-state fermentation (SSF)