Evaluation of Chemical and Biological Characteristics of Subcritical Water Extract from Helicteres isora L. Fruit and Its Incorporation into Chia Seed Mucilage Film

The purpose of this work was to assess the biological characteristic of subcritical water extract of Helicteres isora L fruit. Subcritical water extraction was carried out in particular circumstances (water temperature, flow velocity, pressure, process time, and sample mass were ۱۷۵ºC, ۴ mL/min, ۱۲۰ min, and ۴ g, respectively). Assessment of the chemical composition of H. isora fruit extract affirmed the existence of ۲-propenoic acid, ۲-ethylhexyl ester, heptadecen-(۸)-carbonic acid-(۱), isomenthol, ۱-octen-۳-ol, berberine, ۶,۱۰,۱۴-trimethylpentadecane-۲, docosanoic acid, ۱,۲,۳-propanetriyl ester, phytol, formic acid,۱-methylethyl ester, ۲-methyl-۱-butanol, hexadecanoic acid, and octadecnoic acid. At concentrations higher than ۵۰۰, ۲۰۰, and ۱۰۰ µg/mL, H. isora fruit extract inhibited the growth Escherichia coli, Salmonella enterica, and Pseudomonas aeruginosa, respectively. The AGS cell line was found to be highly susceptible to H. isora fruit extract, with cell viability (%) of ۴۱.۱% at ۱۰۰۰µg/mL. The IC۵۰ magnitude for MKN and AGS (tumor cell lines) were ۵۸.۹ ± ۴.۸ and ۱۲.۳۱ ± ۱.۳ µg/mL, respectively, while the IC۵۰ for the normal cell line (HEK۲۳۹) was ۳۳۵ ± ۲۸ µg/mL. The selectivity indices for AGS and MKN were equal to ۲۷.۲۱ and ۵.۶۸, respectively. Fabrication of active film based on chia seed mucilage and H. isora fruit extract showed inclusion of the extract influenced various properties of the resulted film. Thickness of films enhanced as greater magnitude of the H. isora fruit extract was the added (p<۰.۰۵).  The data depicted that the magnitude of TS and elongation in film samples enhanced as the H. isora fruit extract was incorporated into film formulation (p<۰.۰۵). The analysis of the antioxidant property of fabricated films affirmed that the addition of H. isora fruit extract had remarkably augment the antioxidant property of the film (p<۰.۰۵). The films possess H. isora fruit extract depicted bactericidal characteristic against Escherichia coli and Staphylococcus aureus.

کلیدواژه ها:

Helicteres isora L fruit ، Subcritical water ، Cytotoxicity ، active film