Hemorrhage Control and Wound Healing Using Decellularized Ecm-derived Hydrogel Scaffold Together with Vitamin K

Uncontrolled hemorrhage and infection are the main reasons for many trauma-related deaths in both clinics and battlefields. However, identifying a material with strong biocompatibility and anti-infection properties for skin tissue engineering remains a challenge. Multifunctional hydrogels with excellent adhesion, antibleeding, anti-infection, and wound healing properties are needed. The aim of this study was the use of decellularized extracellular matrix (dECM) with vitamin K for Hemorrhage Control and Wound Healing. In the current study, a novel scaffold was created by combining dECM, alginate, chitosan nanoparticles, and vitamin K. We incorporated vitamin K into the hydrogel to control its release and promote blood coagulation. The scaffolds were characterized by FTIR, mechanical test, swelling, degradation, and scanning electron microscopy (SEM). In addition, cell viability and proliferation were evaluated using MTT assay at ۲۴ and ۴۸ hours as well as SEM imaging. The release assay and coagulation test were also carried out. The results revealed that approximately ۹۸.۱% of immunological components, including cells and DNA, were effectively eliminated from the skin tissue. The dECM/alginate/chitosan nanoparticle/vitamin K composite displayed an interconnected pore structure with a porosity of over ۹۰%, which enhances cell proliferation. The presence of dECM significantly improved cell growth. Including chitosan improved the scaffold by providing strong antibacterial properties, high water, and protein absorption ability to prevent wound infection, and ensuring a balance of moisture and nutrients. The coagulation evaluation results showed that adding vitamin K provided the dECM hydrogels with sufficient coagulation properties. Overall, the dECM/Gel/CS scaffold shows significant promise for use in wound healing due to its suitable mechanical, antibacterial, and coagulant properties with excellent biocompatibility.

کلیدواژه ها:

dECM Hydrogels ، Vitamin K ، Hemorrhage Control ، Wound Healing