Study of Ms c- Gfp + Localization and Fate in Cardiac Micro - Tissue

Introduction Human cardiac microtissues play a significant role in testing regenerative medicine drugs and remodeling cardiovascular diseases. However, cell tracking approaches have rarely been applied to them so far. While the development and maintenance of organs is one of the most fundamental problems in biology, our knowledge of it is still limited. Therefore, in this research, cardiac microtissue consists of three cell sources, including cardiac progenitor cells, mesenchymal stem cells, and human umbilical vein endothelial cells, and our goal in this The project is to investigate the role of mesenchymal stem cells (MSCs) through insert GFP to them, and investigating the fate and location of this cell in the cardiac microtissue. Method In this study, the desigened vectors are purified for virus production by Lenti-x cells. Lipofectamine method is used to trnsfect the packaging cell line. After virus production and transduction, GFP+ mesenchymal cells are cultured together with HUVEC cells which extracted from the umbilical cord and cardiac progenitor cells on rat tail collagen for ۷ days. Microtissue is used for tracking MSC cells for several days. Immunofluorescence was performed to determine the placement of each cell, and RT-PCR was performed to determine the fate of MSC-GFP+ cells. Result In this context, we successfully formed cardiac microtissue through co-vessel in which MSC-GFP+ cells were present and were examined on different days. The result of live and dead heart microtissue shows that a high percentage of cells are alive, and H&E staining was also done to identify the heart tissue. Conclusion MSC-GFP+ cells were seen in the heart microtissue structure, and based on H&E staining, a large number of cells were observed in the heart tissue structure, and the cardiac microtissue was well formed.

کلیدواژه ها:

Mesenchymal stem cells ، self-organization ، cardiac micro-tissue ، co-culture