Methods of Wound-induced in Animal Research on Rats

Wound healing in rats is often used as a preclinical model to study the biological processes involved in tissue repair and regeneration. The rat model provides a controlled environment to observe wound healing mechanisms that are similar to those in humans. Common types of wound healing models in Rats are Incisional, Excisional, Burn, Diabetic, and Ischemic Wound Models. In these models, Wound Closure Rate, Histological Analysis, Biochemical Assays, Immunohistochemistry, and Mechanical Testing were evaluated. In preclinical studies using rats, the number of wounds created on the back depends on the type of wound-healing model, the study design, and the goals of the experiment. Typically, researchers try to maximize the number of wounds while ensuring the health and well-being of the animal and minimizing stress or infection. Usually, ۲ to ۴ full-thickness excisional wounds are created on the dorsal (back) area of the rat. The size of each wound can vary, but a common size is a ۶–۱۰ mm diameter circular wound or a ۱–۲ cm۲ wound. Larger burns may limit the number of wounds due to the increased risk of systemic effects (e.g., infection, dehydration). In diabetic or ischemic rats, wound healing is slower, so researchers may limit the number of wounds to ۱ or ۲ to avoid excessive stress on the animals. There are ethical considerations, and institutional animal care dictates the number of wounds allowed based on the animal’s well-being. Some studies might require more wounds to study different treatments (e.g., using different dressings or drugs on each wound), while others may focus on fewer wounds for more detailed monitoring.

کلیدواژه ها:

wound ، rat ، model