Identification and Isolation of Protease Producing Bacteria From Diabetic Foot Ulcers

This reading was conducted to detect bacterial causative pathogens in patients with diabetes foot ulcer infections and determine the protease enzyme. Samples were collected from ۷۵ swabs were collected from patients suffering from diabetic foot ulcers referred to The Diabetic Center. Data collected from patients included: age, gender and Period of infection. The results revealed that positive bacterial growth appeared in ۵۴ (۷۲ %) of diabetic foot ulcer specimen, and the most common isolates were Enterobacter cloaca ۱۵(۲۷.۷۷%), followed by proteus mirabilis ۱۲ (۲۲.۲۲%), Staphylococcus aureus ۱۰ (۱۸.۵۱%), Escherichia coli ۸ (۱۴.۸۱%), for each one Klebsiella pneumonia, Pseudomonas aeruginosa and Streptococcus pyogens ۳(۰.۸۴%). The results of the Protease enzyme test were performed for all ۵۴ isolates to determine the variable ability of these bacterial species isolated from didactic foot ulcers to produce this enzyme. The highest percentage production protease enzyme was ۱۰۰ % for Pseudomonas aeruginosa, Staphylococcus aureus and ۷۶.۹۲% for proteus mirabilis, while the isolates showed a low production of ۲۰.۰ % for Enterobacter cloaca.

کلیدواژه ها:

Foot ulcer ، proteus mirabilis ، Enterobacter cloaca ، Staphylococcus aureus ، protease enzyme ، infections ، Isolation