Wound Healing Efficacy of Henna (Lawsonia Inermis) Extract in Chitosan and Alginate Natural Polymers

The critical importance of wound healing in human health underscores the pressing demand for developing novel substances with wound-healing properties. The present study aimed to assess the effectiveness of henna (Lawsonia inermis) extract in chitosan and alginate natural polymers for wound healing. In recent years, several methods for synthesizing nanocarriers have emerged. These methods focus on utilizing smart nanocarriers that can respond to their surroundings and deliver their payload in a controlled manner when triggered by internal or external stimuli. In our study, we have successfully created polymeric nanocarriers (PNCs) based on chitosan/alginate loaded with henna extract. The synthesized PNCs were characterized using SEM, DLS, and TEM techniques. The SEM size of the nanoparticles was ۱۰.۳۲ ± ۱.۵ nm. The henna extract content of the PNCs was ۸۹ mg g−۱ with a drug loading efficiency of ۷۱%. The in-vitro cytotoxicity of henna extract, PNCs, and henna extract-loaded PNCs on the HFF-۲ cell line was determined by MTT assay. The IC۵۰ concentrations for henna extract and henna extract-loaded PNCs were ۸۲.۸۶ and ۹۱.۷ µg mL−۱, respectively. Cell migration assay was determined by scratch test on HFF-۲ cell line. Our results showed that henna extract-loaded PNCs prompted cell proliferation and migration capability in ۲۴ and ۴۸ hours. The results suggest that henna extract-loaded PNCs could be a potential source for promoting skin wound healing.

کلیدواژه ها:

Lawsonia inermis ، polymeric nanocarriers ، wound healing