Review the Effect of Lactobacillus Rhamnosus Exopolysaccharide on Pseudomonas Aeruginosa Biofilm Formation

Due to pathogenic bacteria such as pseudomonas aeruginosa have high affinity to forming biofilm, increasing antibiotic resistance inhibition or controlling pathogenicity, dealing with the formation of biofilm with a biological strategy can be considered as a suitable function. In this research, the effective of three different concentrations of ۰.۱, ۰.۵ and ۱ mg/ml of Lactobacillus rhamnosus EPS on Pseudomonas aeruginosa was studied. Probiotics have health effects on humans, as well as parabiotics derived from them with anti-microbial and biofilm. Biofilm can protect bacteria and having antibiotic resistance. So that, Pseudomonas aeruginosa bacteria cause common infections caused by burns and hospital infections by biofilm formation and antibiotic resistance. So that parabiotic effects can prevent the biofilm formation and can be used as a kind of therapeutic supplement along with antibiotics. First, a fresh culture is prepared from Rhamnus and Pseudomonas aeruginosa. for extracting and purifying the exopolysaccharide, the method of Abid et al containing dialysis and multiple centrifuges with combination with ۴% trichloric acid and absolute ethanol are used. To check effect of biofilm formation, two ۹۶-well microplates are used to perform CV and MTT tests. The first plate used for CV test by ۰.۱% dye and the second plate used for second test by MTT salt, with three mentioned concentrations of EPS on ۱۰۰ microliters of Pseudomonas aeruginosa bacteria with concentration of ۰.۵ McFarland with three repetitions for each concentration, after ۷۲ hours of incubation and wash, the reading is done at ۵۹۵ nm for CV and ۵۶۰ nm for MTT (Giordani et al. ۲۰۲۳). The result show that, increasing the concentration of EPS reduces biofilm formation, but it does not reach ۱۰۰% function, and the concentration of ۱ mg has the greatest effect on bacterial biofilm. It can be expected that by increasing the concentration of EPS, more effects on the inhibition of biofilm formation will be included, and also the effect of EPS of different origin can be investigated. Finally, this biological solution can be used along with antibiotics to reduce and inhibit