Research Article: DNA barcoding of wild and culture tilapia based on cytochrome c oxidase subunit I (COI) gene

Tilapia is an introduced fish for aquaculture that spreads across Indonesia’s water resources. The study aimed to determine the mitochondrial DNA COI sequence, the genetic distance and phylogenetic of tilapia from Lake Toba, Lake Ranau, and the Balai Riset Pemuliaan Ikan (BRPI) Sukamandi, West Java. Five individuals were collected from each site, either wild and culture tilapia; strains of blue tilapia and red tilapia resulting from genetic development at BRPI. Basic Local Alignment Search Tool nucleotide (BLASTN) indicated that Oreochromis mossambicus and O. niloticus exist in natural water resources and culture in Sumatra and Java; O. aureus exists in natural water resources of Lake Toba and Lake Ranau, however, O. Urolepsis is only present at the research center of BRPI. The phylogenetic tree indicated four different subclusters of O. niloticus, O. mossambicus, O. aureus, and O. urolepsis; however, all are still in the same cluster with a bootstrap value of ۸۸%.

کلیدواژه ها:

BRPI Sukamandi ، DNA barcoding ، Phylogenetic ، Ranau Lake ، Toba Lake