Provitamin A carotenoid (β-cryptoxanthin) ameliorated testicular ischemia-reperfusion injury in mature rats

Buildup of reactive oxygen species during testicular torsion causes oxidative stress and ischemia-reperfusion (I/R) injury in testis. The purpose of this study was to investigate influence of β-cryptoxanthin (BCX) on I/R injury in testicular torsion/detorsion in mature rats. Thirty mature male Wistar rats were divided into five groups of six animals each, including sham group: In this group, midline incision of the scrotum was performed and the testicles were taken out for ۲ hr with a ۷۲۰-degree rotation, I/R group: In this group, midline incision of the scrotum was performed and the testicles were taken out and undergone ischemia for ۲ hr with a ۷۲۰-degree rotation, I/R/Oil group: In this group, a midline scrotum cut was performed, the testicles were taken out, ischemia was created for ۲ hr with a ۷۲۰-degree rotation, and at the end of ischemia ۱۰۰ µL of corn oil (BCX solvent) was injected intraperitoneally, I/R/BCX۱۰ group: The same as I/R/Oil group, as well as intraperitoneal administration of ۱۰۰ µL of BCX (۱۰.۰۰ µg kg-۱) at the end of ischemia, and I/R/BCX۴۰: The same as I/R/Oil group, as well as intraperitoneal administration of ۱۰۰ µL of BCX (۴۰.۰۰ µg kg-۱) at the end of ischemia. Evaluations were based on histopathological and spermatological parameters and oxidative stress assessments. Histopathological spermatological and oxidative stress parameters values obtained from I/R/BCX۴۰ were significantly different from those of other groups (p < ۰.۰۵). It could be concluded that BCX could ameliorate testicular injuries in acute testicular torsion/detorsion in mature rats.

کلیدواژه ها:

β-cryptoxanthin ، Carotenoid ، Ischemia-reperfusion injury ، Provitamins ، Testis