Rapid extraction and separation of mercury in water and food samples based on micelles and azo-thiazoles complexation before determination by UV-Vis spectrophotometry

A simple and sensitive procedure has been established for analyzing mercury (II) ions spectrophotometrically in the presence of micellar medium using three azo-thiazoles complexing reagents: ۲-amino-۶-(thiazole-۲-yldiazenyl)-۳-pyridinol (C۸H۷N۵OS), ۸-hydroxy-۷-(thiazole-۲-yldiazenyl) quinoline-۵-sulfonic acid (C۱۲H۸N۴O۴S۲), and ۱-hydroxy-۴-(thiazole-۲-yldiazenyl)-۲-naphthoic acid (C۱۴H۹N۳O۳S). H۱ NMR spectra validated the three azo thiazoles synthesized material. Tween ۸۰ (polysorbate ۸۰) and cetyltrimethylammonium bromide (C۱۹H۴۲BrN as molecular biology) are micellar mediums to enhance sensitivity. Absorbances were measured for Hg (II) complexation with R۱, R۲, and R۳ at λmax of ۶۱۷, ۶۳۳, and ۵۵۴ nm, respectively. The UV-Vis spectrophotometer showed calibration curves in the ۰.۲-۱۵ mg L-۱. The molar absorptivity, Sandell's sensitivity, detection, and quantification limits (LOD, LOQ) were determined. The interferences of various ions were investigated, and a statistical assessment of the results was performed. The methods have been applied for trace determination of mercury (II) in food and environmental water samples. For food samples, all samples were digested before complexation with the azo-thiazoles material at optimized pH before determination by UV-Vis spectrophotometry.

کلیدواژه ها:

Mercury ، UV-Vis spectrophotometry ، Ligand ، Azo-thiazoles ، Complexation ، Water and Food samples