The effect of different concentrations of auxin and cytokinin on the in-vitro propagation of Insulin plant (Chamaecostus cuspidatus Nak)

The anti-diabetic medicinal plant of Chamaecostus cuspidatus Nak is an important medicinal plant due to having an extensive range of bioactivities, including anti-diabetes, anti-cancer and anti-inflammatory. This research investigates the effects of different concentrations of growth regulators, namely NAA and BA, on nodal explants in two separate experiments on plant micropropagation. This experiment was conducted using a factorial in a completely randomized design (CRD). The Hormonal concentrations in the first experiment include treatments of BA (۰, ۲, ۴ and ۶ mg/L-۱) with NAA (۰, ۰.۳ and ۰.۶ mg/L). The results of the first experiment led to the design of the second experiment with optimal concentrations. The second experiment included BA (۰, ۱, ۲ mg/L-۱) with NAA (۰, ۱.۲, ۲.۴ mg/L-۱) in MS culture medium. The results of the first experiment showed that the highest Percentage of shoot induction, number of leaves, plant height, shoot fresh weight, Percentage of root induction, root fresh weight, root diameter and photosynthetic pigments among different treatments related to zero BA treatment with ۰.۶ mg/L NAA and The lowest value corresponds to the control. The results of the second experiment showed that the culture medium containing ۲.۴ mg/L of NAA along with ۱ mg/L of BA had the greatest effect on seedling growth and shoot growth. The highest root length (۲۰.۳۳ cm) was obtained in the medium under treatment with ۱.۲ mg/L of NAA with zero and ۱ mg/L BA and ۲.۴ mg/L of NAA with ۱ and ۲ mg/L BA. Additionally, the index of chlorophylls showed a direct relationship with the growth of aerial organs and proper propagation, so that the amount of chlorophylls increased with the growth of the plant (۱ mg/L BA with ۲.۴ mg/L NAA). The present study demonstrated that the plant requires a small amount of the hormone BA (۱ mg/L) for enhanced shoot growth. On the other hand, increasing the amount of NAA from ۱.۲ mg/L to ۲.۴ mg/L did not show a significant difference in root growth, and at a concentration of ۲.۴ mg/L, the plant exhibited callus formation.

کلیدواژه ها:

BA ، Medicinal plant ، Micropropagation ، NAA ، Plant growth regulator ، Plant tissue culture ، تنظیم کننده رشد گیاه ، ریزازدیادی ، کشت بافت گیاهی ، گیاه دارویی ، BA ، NAA