The Impact of Gold Nanoparticles on the Expression of Several Epigenetic Genes in the Human Renal Cell Carcinoma A۴۹۸ Cells

In this work, walnut was used to create gold nanoparticles, and their effectiveness against kidney cancer cells in vitro was evaluated. UXRD and TEM examination were used to analyze the biosynthesized walnut gold nanoparticles (WGNP). The MTT assay was used to evaluate the cytotoxicity against the renal cancer cell lines A۴۹۸ and SW-۱۵۶. The real-time PCR technique was used to determine the fold changes of NANOG, OCT۴, SOX, P۵۳, and CAS expression against β-actin. Two-Way ANOVA was used to evaluate the data. If the difference was less than ۰.۰۵, it was deemed significant.