Synthesis of CNT/PEG magnetic nanoparticles for stabilization and immobilization of laccase enzyme

Laccases are a family of copper-containing polyphenol oxidases that belongs to the multicopper oxidases. The MNPs can be remotely controlled by magnetic fields, which make it possible for magnetic separation and targeted drug delivery. Immobilization essentially attaches or wraps free enzymes to a specific support by physical or chemical means to retain or improve their practical properties. Polyethylene glycol (PEG) is a type of polymer material that is attached to the surface of nanoparticles by a stable bond. FeCl۲, FeCl۳ and ۳۰% NaOH were used for the synthesis of magnetic nanoparticle with PEG and CNT coating, then maleic anhydride was functionalized and laccase enzyme was immobilized on it using EDAC and NHS linkers. Free and immobilized enzyme activity was measured between ۳۰ and ۷۰°C. Laccase-stabilized nanoparticle still retains its magnetic property. The stabilization percentage was ۵۶% and the optimal temperature of free and stabilized enzyme is ۳۵°C. The enzyme was successfully immobilized on the nanoparticle and showed better stability than the free enzyme in the temperature test. It was shown that the enzyme immobilized on nanoparticles can be most effective in industrial applications.

کلیدواژه ها:

Laccases ، Polyethyleneglycol ، Carbon nanotubes ، Nanoparticle ، immobilization