The ameliorative effects of hesperidin in rats developed hepatotoxicity with deltamethrin

Objective(s): Deltamethrin (DLM) is a widely used insecticide in agriculture; however, exposure to it can lead to serious health problems. This study aimed to evaluate the protective effects of hesperidin (HSP), a natural antioxidant, against DLM-induced liver toxicity.Materials and Methods: Thirty-two male Wistar Albino rats (۲۵۰–۳۰۰ g, ۴ months old) were divided into four groups. The control group received ۱ ml of corn oil via oral gavage for ۳۰ days. The DLM group received ۱.۲۸ mg/kg DLM in corn oil for ۳۰ days. The DLM+HSP ۱۰۰ mg/kg and DLM+HSP ۳۰۰ mg/kg groups received ۱.۲۸ mg/kg DLM followed by ۱۰۰ mg/kg or ۳۰۰ mg/kg HSP in distilled water, respectively, ۳۰ min after DLM administration for ۳۰ days. Liver tissues were examined histopathologically. Masson’s trichrome staining and PCR assessed fibrosis. Caspase ۳ and ۹ expressions in liver tissues were determined by immunohistochemistry and PCR. Biochemical analyses were conducted on serum samples.Results: HSP supplementation led to a dose-dependent decrease in aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels. DLM exposure decreased antioxidant capacity, while HSP supplementation increased it dose-dependently. Histopathological evaluations showed increased liver damage in the DLM group, while HSP administration reduced liver toxicity. Masson’s trichrome staining and analysis of collagen I (COL۱A۱) and collagen III (COL۳A۱) gene expression revealed increased fibrosis in the DLM group, which was attenuated with HSP treatment.Conclusion: The potential prevention of DLM-induced liver toxicity and apoptosis by HSP may be an alternative protective strategy.

کلیدواژه ها:

Classical Apoptosis ، Hesperetin- ۷-Rhamnoglucoside ، Insecticides ، Oxidative stresses ، Toxicity