The Application of Multiplex Real-Time PCR to Detect High-Risk HPV in Iraqi Population

Background and Objective: Cervical cancer stands as the leading cause of cancer-related fatalities, worldwide. Cervical cancer is unequivocally attributed to the human papillomavirus (HPV) infection, which is both essential and adequate for its development. The particular prevalence of HPV in the population of Iraq is currently unclear. The present study was to examine the application of multiplex real-time PCR to detect high-risk HPV in the Iraqi population. Methods: This study was conducted on a total of ۳۰۰ women with cervical anomalies, who were referred to the Gynecological Oncology Departments in multiple hospitals in Babylon and Baghdad from November ۲۰۲۲ to December ۲۰۲۳. The cervical swabs of the samples were collected. Real-time polymerase chain reaction was utilized to conduct HPV DNA testing on all specimens.  Result: The average age of the participants was ۳۰ years. The overall prevalence of High-risk HPV was ۱۶.۷%. The age group of women between ۳۰ and ۴۰ years exhibited the highest overall prevalence of HPV. There was a higher prevalence of genotyping for HPV ۱۶, ۱۸, and ۳۱. Conclusion: The multiplex real-time PCR has numerous advantages, including HPV detection and genotyping to known prevalent genotypes as well as cost effective and no time consuming.

کلیدواژه ها:

Carcinoma ، cervical cancer ، HPV ، Oropharyngeal Malignancies ، STDs