Gut Proteomics Reflecting Extraintestinal Organ Involvement in IBD

Inflammatory Bowel Disease, including Crohn's Disease (CD) and Ulcerative Colitis (UC), is a chronic gastrointestinal disorder characterized by inflammation. About ۲۵% of IBD patients are pediatric (Rosen et al., ۲۰۱۵). Pediatric IBD is of concern due to aggressive progression, nutritional deficiencies, growth failure, and complications from early disease onset (Ishige, ۲۰۱۹). Additionally, pediatric IBD care is more costly than adult care (Kappelman et al., ۲۰۰۸). IBD affects not only the gastrointestinal tract but also other organs, referred to as extraintestinal complications. These can involve the musculoskeletal system, skin, hepatobiliary tract, and eyes, significantly impacting patients' quality of life (Rogler et al., ۲۰۲۱). Also, extraintestinal complications present challenges for healthcare providers in managing IBD. We employed proteomics data from a cohort of pediatric patients, consisting of ۲۲ CD cases, ۱۸ UC cases, and ۲۲ healthy controls from mucosal luminal interface samples of the colon (Zhang et al., ۲۰۱۸). Raw MS data were preprocessed using MaxQuant and differentially expressed proteins among CD, UC, and healthy controls were identified using the DEP package in R. We focused on proteins significantly altered in IBD patients compared to healthy controls as well as those distinguishing subtypes of IBD. Enrichment analysis was conducted to identify tissues and gene ontologies associated with the significant proteins. Our findings suggest that intestinal proteomics data reflects the potential links between other organs and IBD, which may offer insights into the broader physiological effects of the condition.

کلیدواژه ها:

pediatric inflammatory bowel disease (PIBD) ، Extraintestinal Organ Involvement ، Proteomics ، Differentially Expressed Proteins ، Enrichment Analysis