Identification of circRNA-miRNA-mRNA Interaction in Myocardial Infarction

Myocardial infarction (MI) is a severe cardiovascular condition resulting from sudden blood flow blockage to a heart muscle, causing tissue damage and severe patient outcomes, prompting researchers to explore molecular mechanisms. Non-coding RNAs, such as miRNAs and circRNAs have recently gained attention due to their regulatory roles in gene expression. Emerging evidence suggests that non-coding RNAs play crucial roles in cardiovascular diseases, including MI. Gene expression profiles (GSE۲۴۵۱۹ and GSE۲۴۵۴۸) were downloaded from the Gene Expression Omnibus. Differentially expressed genes and miRNA were identified using the GEO۲R. Enrichr was used to obtain the pathway and biological process. miRTarbase and CircBank databases were used to retrieve miRNAs and circRNAs. The circRNA-miRNA-mRNA interaction was reconstructed with Cytoscape software. Functional enrichment analysis revealed that several pathways and biological processes associated synthesis of PIPs at early endosome membrane, PI metabolism, adherens junctions interactions, protein deglutamylation, receptor catabolic process and cellular response to potassium ion. The results of this study showed that ZNF۷۰۱, HOXA۱۰, TSR۱, miR-۴۸۵-۵p, miR-۱۴۲-۳p, miR-۱۴۲-۵p, circ_۰۰۸۷۴۲۴, circ_۰۰۸۷۴۲۵, circ_۰۰۸۷۴۲۷ and circ_۰۰۸۷۴۲۸ have the most important regulatory role. This study identifies a circRNA-miRNA-mRNA interaction, offering insights into MI pathophysiology and potential biomarkers for managing this serious cardiac condition.

کلیدواژه ها:

circRNA ، miRNA ، myocardial infarction ، regulatory network