In Silico Design of a Chimeric Protein-Based Vaccine Against Salmonella Typhi: A Bioinformatics and Immunoinformatics Approach

Salmonella is a Gram-negative, rod-shaped bacterium from the family Enterobacteriaceae. Serovar Typhi causes human typhoid fever that leads to systemic infection in the host. This disease is treated using broad-spectrum antibiotics. The emergence of multidrug-resistant bacteria has highlighted the urgent need for developing antibiotic-independent therapeutic approaches and prevention strategies through vaccines. Chimeric proteins are well-suited for eliciting cell-mediated immunity, resulting in adequate immunogenicity against S. Typhi. Two antigens, T۲۹۴۲ and FliC, were selected as candidates for the design of a vaccine. The protein sequences of these proteins were connected using a rigid linker and subjected to detailed bioinformatics and immunoinformatic analyses. Phyre۲ predicted the three-dimensional structure, and SAVES evaluated biophysical and biochemical parameters. Immunoinformatic evaluations were performed to assess the protein's immunogenicity and non-allergenic properties. The results demonstrate the stability of the chimeric protein and its ability to elicit an immune response in the host.

کلیدواژه ها:

Salmonella Typhi ، chimeric protein ، vaccine ، bioinformatics ، immunoinformatics