Design and Analysis of siRNA for Silencing the NS۳ Gene of Hepatitis C Virus: A Novel Therapeutic Approach

This study focuses on the design and analysis of siRNA to inhibit the NS۳ gene of the Hepatitis C Virus (HCV). The main objective was to identify mRNA sequences of the NS۳ gene and design siRNAs to suppress its activity. Using NCBI, the NS۳ gene sequence was identified, and the coding sequence of the helicase domain was selected due to its conserved nature for siRNA design. RNAstructure software was employed to simulate siRNA-mRNA interactions for the HCV genome. Additionally, BLAST and RNAhybrid tools were used to evaluate potential off-target effects on the human genome. These methods quantitatively predicted the targeting accuracy and efficacy of siRNAs in inhibiting HCV replication. The results demonstrate that the designed siRNAs exhibit significant potential as an effective therapeutic strategy for treating HCV infection.

کلیدواژه ها:

siRNA design ، Hepatitis C Virus (HCV) ، NS۳ helicase inhibition ، RNA interference (RNAi) ، therapeutic RNA silencing