The Association Between RACK۱ Gene Polymorphisms rs۱۲۷۹۷۳۶ and rs۳۷۵۶۵۸۵ in Uterine Cervical Cancer with the Risk Factor of HPV: A Focus on Squamous Cell Carcinoma (SCC)

Background & Objective: Cervical cancer is the second most common malignancy among women worldwide. The Receptor for Activated C Kinase ۱ (RACK۱) has a key role in regulating the pathways of cell signaling, angiogenesis, cell migration, invasion, and metastasis. This study investigated the association of polymorphisms rs۱۲۷۹۷۳۶ and rs۳۷۵۶۵۸۵ with cervical cancer.Methods:  In this study, ۱۰۰ LB pap-smear and paraffin block samples of cervical squamous carcinoma associated with Human papillomavirus (HPV) were selected along with ۱۰۰ LB smear cytology samples from healthy women with normal pap smears, serving as the control group. Genomic DNA was extracted from the samples, and the polymorphisms rs۱۲۷۹۷۳۶ and rs۳۷۵۶۵۸۵ in the RACK۱ genes were examined using the RFLP-PCR and ARMS-PCR methods, respectively.Results: The rs۱۲۷۹۷۳۶ polymorphism shows that the chance of cervical cancer in individuals with CA and AA genotypes is ۳.۷۷۲ and ۱.۸۵۲ times that of CC genotypes, respectively. This suggests that the A allele is involved in developing cervical cancer (P=۰.۰۰۵; OR=۲.۱۱۳). The rs۳۷۵۶۵۸۵ polymorphism indicates that the chance of cervical cancer in TG and GG genotypes is ۰.۳۷۳ and ۴.۲۳۵ times that of TT genotypes, respectively. This also points towards the involvement of the G allele in the development of cervical cancer (P=۰.۰۳۲; OR=۱.۸۰۰). Also, there was no significant relationship between the genotype of polymorphisms, age, and subtype of papillomavirus ۱۶ and ۱۸ in patients.Conclusion: The present study was conducted for the first time. Our results showed that two polymorphisms are significantly associated with cervical cancer.

کلیدواژه ها:

cervical cancer ، HPV ، Genetic polymorphisms ، RACK۱