A Sensitive Colorimetric Assay for Acetylcholinesterase Activity based on Aggregation of Gold Nanoparticles with Phosalone

A highly sensitive, fast and convenient colorimetric assay for acetylcholinesterase (AChE) activity was developed. To do this, phosalone was used to aggregate citrate-coated gold nanoparticles which caused its color to be changed from wine-red to blue. This aggregation was significantly hindered with AChE, due to its preferential reaction with phosalone, and the corresponding color change was probed spectrophotometrically as a function of AChE activity. The ratio of the measured absorbances at the wavelengths of ۵۲۰ and ۶۵۰ nm (A۵۲۰/A۶۵۰) was found to be linear with the activity of AChE from ۰.۰۵۰ to ۱.۰ U mL−۱ (R۲ = ۰.۹۹۱۲). A limit of ۰.۰۳۰ U mL−۱ for detection of AChE was achieved based on signal to noise ratio of ۳. The procedure was successfully applied for determination of AChE in real samples as well as quantitation of phosalone in diverse fields such as food and agricultural industries as well as pharmaceutical, healthcare and environmental affairs.

کلیدواژه ها:

Acetylcholinesterase ، Phosalone ، Gold Nanoparticles ، Aggregation ، Colorimetric assay