Exploring the phylogenetic group and clonal relationship Escherichia coli species obtained from hedgehogs in Kerman, Iran

ERIC-PCR technique is a simple, fast and cost-effective genotyping technology to detect different types of Escherichia coli species and genetic fingerprinting. The present study was designed and carried out with the aim of determining the phylogenetic group and clonal relationship of Escherichia coli isolates obtained from hedgehogs. In the present study, ۲۰ hedgehog feces samples were collected in Kerman city within ۱ month and immediately sent to the veterinary faculty laboratory in less than ۲۴ hours, and with the help of culture methods, one Escherichia coli isolate was obtained from each sample. After DNA extraction, PCR test was performed to trace the phylogenetic group of Escherichia coli isolates and to check the clonal relationship of the isolates by ERIC-PCR method. In this study, among ۱۰۰ isolates, ۴۳ isolates belonged to phylogenetic group A, ۵۲ isolates belonged to a phylogenetic group B۱, ۳ isolates belonged to group D, and ۲ isolates were undetermined. Also, based on ERIC-PCR, these ۱۰۰ isolates were grouped into ۳۱ ERIC types with more than ۹۸% similarity. Also, the strains in the porcupine population studied, which were related to a specific and limited geographical area, have a significant similarity to each other. Escherichia coli strains isolated from hedgehogs, have been shown to be mostly non-pathogenic in nature.

کلیدواژه ها:

Escherichia coli ، Phylogeny ، ERIC-PCR ، Kerman ، Iran.