Impact of Human Umbilical Cord Mesenchymal Stem Cell Conditioned Medium on the Secretion Profile of Human Liver Cancer Cell Line HEPG۲

Background and aim: Cell therapy and gene therapy have shown promise in enhancing normal liver function and providing a bridge to organ transplantation. Hepatocellular carcinoma (HCC) has doubled in incidence over the past decade, exacerbating the disease burden. Early detection and monitoring of HCC are crucial due to its rapid progression. Identifying sensitive indicators for early detection of individuals at high risk of developing HCC can reduce mortality and medical costs. This study aimed to investigate the impact of purified media from Wharton's jelly-derived mesenchymal stem cells on the human liver cancer cell line HepG۲. The evaluation focused on the biochemical functions. Methods: Enzyme-linked immunosorbent assay (ELISA) is a common laboratory method for the detection and quantification of proteins, antibodies, or small molecules. ELISA test is usually used to detect antigen or antibody. In this way, one of these two substances is fixed in the solid bed and used to track the second one. The concentration of PIVKAII, AFP-L۳, AFP in different groups was measured by chemiluminescent magnetic microparticle immunoassay (CMIA) according to the instructions of the protocol kit (Abbott. Laboratories, Sligo, Ireland). Results: The results of the study demonstrated that conditioned environments exert a significant effect on the secretion of these substances from HepG۲ cells. Notably, the secretion of alpha-fetoprotein, inducible protein in the absence of vitamin K, liver enzymes, and bilirubin decreased significantly in the presence of purified media. Conversely, the secretion levels of these proteins increased when exposed to refined environments. Conclusion: In conclusion, the findings provide critical insights into the impact of human umbilical cord mesenchymal stem cell conditioned medium on the secretion profile of the human liver cancer cell line HEPG۲. This research offers a deeper understanding of the potential effects of mesenchymal stem cells on liver