The Effect of Static Magnetic Field on the Rate of proIAPP Amyloid Structures Formation and the Toxicity of Amyloid Structures of Lysozyme

Aims: Living cells have an electrical charge created by the presence of ions and free radicals. Magnetic fields interact with ions, especially ferromagnetic materials such as iron that affect living cells. A common feature of about ۲۰ different diseases is the aggregation of proteins in the form amyloid structure. In the current study, the effect of static magnetic field (SMF) on the formation and the toxicity of amyloid structures was investigated. Materials & Methods: CHO cells were exposed to ۶mT SMF in three consecutive days, and the effect of SMF on the formation of amyloid structures in the intrinsic proteins of these cells related to the control was investigated using thioflavin T (ThT) binding assay. The formation of amyloid structures in CHO cells expressing human ProIAPP cells was analyzed by observation of proIAPP protein aggregates linked to GFP protein. The effect of SMF on the toxicity of lysozyme oligomers on CHO and Hela cells was also compared with the controls. Findings: Exposure of CHO cells to magnetic fields does not have a significant effect on the formation of amyloid structures in the intrinsic proteins of CHO cells and the amount of these structures in CHO cells expressing proIAPP protein but can increase the toxicity of lysozyme oligomers on CHO and Hela cells. Conclusion: The magnetic field does not have a significant effect on the formation of amyloid structures in the ۶mT SMF strength, but it adds to the toxicity of these structures.

کلیدواژه ها:

Static Magnetic Field ، Lysozyme ، Islet Amyloid Polypeptide ، میدان مغناطیسی ایستا ، لیزوزیم ، پلی پپتید آمیلوئیدی جزیره ای