Evaluation and Comparison of New Methods in HLA Typing

The human leukocyte antigen (HLA) system has unique genetic and biochemical complexes, so it is important to determine the type of HLA in a clinical and laboratory process. In addition, to its application to assess the risk of various diseases, it also plays a vital role in organ transplants. The determination of HLA in patients requiring transplantation, in addition to the accuracy, also requires the speed of action. So that any delay and error in this work can endanger the patient's life. The need for a precise definition of the HLA type, along with improved speed and cost reduction, has led to the introduction of several methods for this test. Today, the introduction of a new generation of revolutionary sequencing has been carried out in genetic assessments. The HLA determination also uses this technique, and a variety of methods have been introduced for genetic HLA examination using a new generation of sequencing. In the present study, along with the review of traditional methods in HLA diagnosis, two of the most widely used techniques based on a new generation of sequencing methods, Thermal Sequencing method and Illumina Miseq method were evaluated.

کلیدواژه ها:

Major Histocamplimentary System (MHC) ، Human Leukocyte Antigen (HLA) ، Next Generation of Sequencing ، Ilumina Miseq ، Thermal Sequence Detection ، سیستم سازگاری نسجی انسان ، آنتی ژن های لکوسیت انسانی ، نسل جدید تعیین توالی ، ایلومینا Miseq ، تعیین توالی حرارتی