Design, Expression, Purification, and Evaluation of Anti-HER۲ scFv

Aims: The aim of the present study was to design and express an anti-HER۲ single chain variable antibody fragment in E. coli BL۲۱ (DE۳) and evaluate its efficiency in recognition of HER۲ protein. Materials & Methods: An approximately ۷۴۶bp encoding gene fragment was cloned into pET۲۸a and the recombinant protein was expressed in E. coli BL۲۱ (DE۳) strain. Following protein purification by affinity chromatography, western blotting and ELISA were used to evaluate the efficiency of anti-HER۲ scFv against HER۲ protein. Findings: E. coli can express the anti-HER۲ scFv molecule possessing appropriate function and can detect this protein on the surface of breast cancer cells. Conclusion: This antibody fragment can be used in laboratory diagnostic methods for HER۲ diagnostic approaches. Potential capability of this protein in immunohistochemical and imaging approaches against HER۲ should be considered.

کلیدواژه ها:

Single Chain Antibody Fragment ، HER۲ Protein ، Cell-Based ELISA ، قطعه آنتی بادی تک زنجیره ، پروتئین HER۲ ، الایزای سلولی