Isolation and Identification of Halophilic Bacteria Producing Asparaginase Enzyme from Urmia Lake

Aims: The present study was aimed to investigate isolation and identification of halophil bacteria producing asparaginase from Urmia Lake and the effect of different salt concentrations and temperature on asparaginase production. Materials & Methods: The water and mud samples from Urmia Lake were cultivated in MH and SWN media for isolation of halotolerant bacteria. Then, the asparaginase production rate was investigated in saline M۹. The asparaginase production rate by isolates was investigated in different salt concentrations (۱۰, ۲۰, ۳۰, and ۴۰%) and temperatures (۳۰, ۳۵, ۳۷, ۴۵, and ۵۰°C). Two isolates with a high production rate of asparaginase were stained by gram stain method and were identified by ۱۶S rRNA sequencing. Findings: In the present study, ۵۷ isolates were obtained which ۱۰ isolates showed asparaginase production. Among these, ۲ isolates (A۲ and A۳) were selected as superior isolates. The isolate A۳ has shown the largest halo (۵۴mm) at ۳۵°C and ۱۰% salt concentration. Moreover, the isolate A۲ has shown the largest halo (۵۶mm) at ۳۵°C and ۴۰% salt concentration. The ۱۶SrRNA sequencing showed that the A۲ isolate was similar ۹۸.۹% to the Halomonas elongata strain, and the A۳ isolate was similar ۱۰۰% to the Bacillus aryabhattai strain. Conclusion: The bacteria isolated from Urmia Lake are potential source of asparaginase enzyme. H. elongate and B. aryabhattai strains isolated from Urmia Lake can be used to production of asparaginase without side effects for cancer patients.

کلیدواژه ها:

Asparaginase ، Halophile ، Therapeutic Uses ، Urmia Lake ، آسپاراژیناز ، کاربردهای درمانی ، نمک دوست ، دریاچه ارومیه