Investigation of Combination Effect of Doxorubicin and Zinc Oxide Nanoparticles on the Expression of Long Non-Coding RNA INXS in MCF-۷ Breast Cancer Cells

Aims: The combined therapy of cancer is more effective than using a single drug to treat. In the present study, the anticancer effects of doxorubicin and doxorubicin in combination with zinc oxide nanoparticles on the MCF-۷ breast cancer cells were investigated. Materials & Methods: MCF-۷ cells were cultured in RPMI۱۶۴۰ medium. The number of viable cells and the activity of mitochondrial dehydrogenase enzyme were evaluated by MTT assay for ۲۴, ۴۸, and ۷۲ hours and IC۵۰ was determined. RNA was extracted and then cDNA was synthesized. Specific primers for the INXS and TBP genes were designed using proprietary software. The expression level of INXS gene compared with TBP reference gene using Real-Time PCR method was assessed. Findings: The expression of the INXS gene in doxorubicin-treated MCF-۷ cells at ۲۴, ۴۸, and ۷۲ hours was ۷.۹۳, ۱.۷۱, and ۰.۱۹, respectively, and in doxorubicin-treated cells with zinc oxide nanoparticles was changed ۱.۸۱, ۶.۳۴, and ۱۵.۹۶ at ۲۴, ۴۸, and ۷۲ hours, respectively. Conclusion: Simultaneous treatment with doxorubicin and zinc oxide nanoparticles at low concentrations (۳.۱۲۵ and ۶.۲۵) can cause more cell death than doxorubicin treatment alone. This may be due to the facilitation of doxorubicin entry into the cell in the presence of zinc oxide nanoparticles.

کلیدواژه ها:

Doxorubicin ، Zinc Oxide Nanoparticle ، Breast Cancer ، INXS ، LncRNA ، دکسوروبیسین ، نانوذره اکسید روی ، سرطان پستان ، INXS ، LncRNA