Comparison of Humoral and Cell-Mediated Immune Response to Tetanustoxin Coated PLGA in Mice

Aims: New vaccines based on recombinant and DNA proteins are safer than traditional vaccines, but unfortunately, they have lower Therefore, there is a need for the development of safe and strong that can increase the immune PLGA), ester, consists of acidic and lactic acid. Its hydrolysis leads to the production of lactic acid and glycolic acid monomers. The aim of this study was to compare humoral and cell mediated immune response to coated PLGA in mice. Materials and Methods: In this experimental study, PLGA nanoparticles were produced by water/oil (W/O) method. Tetanus toxin attached to by EDC. After coated characterization, they were injected into different groups of mice. The complete and Alum as After a single injection, the of was investigated by ELISA and cellular analyzed by spleen cell proliferation assay. One-way analysis of variance was used. Findings: PLGA nanoparticles had a strong effect, and when used with antigens, could produce cellular and humoral immune response far more powerful than alum and than Freund’s adjuvant. Conclusion: Glycolic polyester, in the form of conjugation with an antigen, can be used to increase the immune response, especially in the cellular immune arm, relative to the antigenic solution. Although PLGA seems not so successful to the humoral immune stimulus against in comparison to the full of it can be a significant competitor with

کلیدواژه ها:

Nanoparticles ، PLGA ، Tetanus Toxin ، Immune response ، Adjuvant ، نانوذرات ، PLGA ، توکسین تتانی ، پاسخ ایمنی ، ادجوانت