Study of Protective Effect of Nigella Sativa Nanocapsules on Tissue Pathology and IL-۱۰ levels in Lung Tissues Exposed to NNK

Aims: It is assumed that ketone derived from tobacco (NNK) which is the most important carcinogen in tobacco modulates alveolar macrophage mediator production, such as anti-inflammatory cytokine IL-۱۰. The aims of this study were to investigate the effect of a Nigella sativa on tissue pathology and IL-۱۰ levels in lung tissues exposed to NNK Materials & Methods: In this experimental study, ۴۶ Wistar rats of supplement, supplement+NNK, NNK, solvent and control. NNK-induced groups received NNK subcutaneously one day per week at a rate of ۱۲.۵ mg per kg body weight. Supplemented groups also consumed Nigella sativa Nanocapsules for ۱۲ weeks. Levels of IL-۱۰ in homogenized lung tissue were measured by ELISA. To analyze the data; ANOVA and Tukey test were used at a significance level of p≤۰۰۵. Findings: Exposure to NNK increased levels of IL-۱۰ compared with the solvent group, although not statistically significant (p≥۰۰۵). Meanwhile, a period of consumption of Nigella sativa Nanocapsules significantly increased levels of IL-۱۰ in NNK + supplement  and the supplement groups compared to NNK group (p=۰.۰۳۸, p=۰.۰۰۲; respectively). In addition, the effect of Nigella sativa Nanocapsules on IL-۱۰ levels of lung tissue was shown that the levels of this variable were significantly higher than the solvent group (p=۰.۰۰۱). Conclusion: Generally it could be confirmed that consumption of Nigella sativa Nanocapsules plays an important role in the inhibition of inflammation induced by NNK via Increase of IL-۱۰ activity. Aims: It is assumed that ketone derived from tobacco (NNK) which is the most important carcinogen in tobacco modulates alveolar macrophage mediator production, such as anti-inflammatory cytokine IL-۱۰. The aims of this study were to investigate the effect of a Nigella sativa on tissue pathology and IL-۱۰ levels in lung tissues exposed to NNK Materials & Methods: In this experimental study, ۴۶ Wistar rats of supplement, supplement+NNK, NNK, solvent and control. NNK-induced groups received NNK subcutaneously one day per week at a rate of ۱۲.۵ mg per kg body weight. Supplemented groups also consumed Nigella sativa Nanocapsules for ۱۲ weeks. Levels of IL-۱۰ in homogenized lung tissue were measured by ELISA. To analyze the data; ANOVA and Tukey test were used at a significance level of p≤۰۰۵. Findings: Exposure to NNK increased levels of IL-۱۰ compared with the solvent group, although not statistically significant (p≥۰۰۵). Meanwhile, a period of consumption of Nigella sativa Nanocapsules significantly increased levels of IL-۱۰ in NNK + supplement  and the supplement groups compared to NNK group (p=۰.۰۳۸, p=۰.۰۰۲; respectively). In addition, the effect of Nigella sativa Nanocapsules on IL-۱۰ levels of lung tissue was shown that the levels of this variable were significantly higher than the solvent group (p=۰.۰۰۱). Conclusion: Generally it could be confirmed that consumption of Nigella sativa Nanocapsules plays an important role in the inhibition of inflammation induced by NNK via Increase of IL-۱۰ activity.

کلیدواژه ها:

Nigella sativa ، Nanocapsules ، IL-۱۰ ، NNK ، Histologic Structure ، نانوکپسول سیاه دانه ، اینترلوکین ۱۰ ، NNK ، ساختار هیستولوژیک