Anti-angiogenesis Effect of C-Phycocyanin of Spirulina platensis on B۱۶-F۱۰ Melanoma Tumors in C۵۷BL/۶ Mouse

Aims: Melanoma is one of the most dangerous forms of skin cancer, which is unresponsive to the current chemotherapy drugs. As a natural product purified from spirulina, phycocyanin can inhibit the angiogenesis. The aim of this study was to investigate the anti-angiogenesis effect of C-phycocyanin of spirulina platensis on B۱۶-F۱۰ melanoma tumors in C۵۷BL/۶ mouse. Materials and Methods: In this experimental study, ۱۶ C۵۷BL/۶ mice with the age range of ۶-۸ weeks were randomly divided into two groups, including control and phycocyanin groups. On the day ۰ of the study, melanoma cells were injected and all the mice were treated for ۲۰ days. Phycocyanin group received ۴۰mg/kg phycocyanin every day. The tumors were extracted on the day ۲۱ and the effect of phycocyanin on the angiogenesis and proliferation of cancer cells was investigated, using immunohistochemical staining with CD۳۱ and Ki-۶۷, respectively. The data were analyzed, using JMP ۱۱ software by one way ANOVA test. Findings: In the phycocyanin group, angiogenesis was significantly lower than that of the control group (p<۰.۰۱), while the mitotic index was not significantly lower than that of the control group in the mice treated with phycocyanin. Conclusion: Phycocyanin has ability to inhibit angiogenesis in the B۱۶-F۱۰ melanoma tumors in C۵۷BL/۶ mouse, but it is not able to reduce the proliferation of melanoma cells.

کلیدواژه ها:

Phycocyanin ، Angiogenesis ، Ki-۶۷ ، Melanoma ، Spirulina platensis ، فیکوسیانین ، رگ زایی ، Ki-۶۷ ، ملانوما ، اسپیرولیناپلاتنسیس