Assessment of anticancer properties of Rosmarinus officinalis L extract and gamma rays on cell viability of MCF-۷, SKBR۳, and HU۰۲ cell lines

Objective: Despite significant advances in radiation therapy and cancer treatments in the past ۳۰ years, resistance to chemotherapy is a major obstacle in the recovery of patients with cancer. Resistance to chemotherapy drugs inhibits the recovery process. This study aims to evaluate the anticancer activity of the methanolic extract of rosemary alone and in conjunction with gamma rays on growth inhibition of MCF-۷,SKBR۳ and Huo۲ cell lines. Methods: We examined cytotoxicity of different concentrations (۱, ۵, ۱۰, ۵۰, ۱۰۰, ۵۰۰ μg/ml) of rosemary extract and various doses of gamma rays (۱.۵, ۳, and ۷.۵ Gy) on MCF-۷, SKBR۳, and fibroblast (HU۰۲) cell lines. All three cell lines were obtained from the Iranian Biological and Genetics Reserve Center. The cell lines were grown in DMEM supplemented with ۱۰% FBS, ۱% penicillin and streptomycin. The cells were allowed to incubate at ۳۷ºC in an atmosphere that contained ۵% CO۲ and ۱۰۰% humidity. The standard MTT assay was performed to estimate cell viability after treatment with gamma rays and rosemary extract. Results: The results of the MTT assay showed that rosemary extract had time- and concentration-dependent anticancer activities on the MCF-۷ and SKBR۳ cell lines (p<۰.۰۱). Rosemary extract had no remarkable cytotoxicity on the normal HU۰۲ cell line. Gamma rays along with rosemary extract had more cytotoxic activity in a time-dependent manner on viability in the cancer call lines (p<۰.۰۳). Conclusion: Our results, along with results from other studies, have suggested that rosemary extract is a potential candidate, either alone at pre-determined doses or in combination with gamma rays, for the treatment of chemotherapy resistant breast cancer.

کلیدواژه ها:

Rosemary extract ، Gamma rays ، Cytotoxicity ، عصاره رزماری ، تابش گاما ، سمیت