Investigation of morphological behavior of large vessel human endothelial cells during radiation therapy based on cytoskeleton imaging

سال انتشار: 1396
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 76

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شناسه ملی سند علمی:

JR_PRJMS-20-2_005

تاریخ نمایه سازی: 28 اسفند 1403

چکیده مقاله:

Objective: Pathological changes to endothelial cells of the vessel wall may lead to vascular stenosis. In this study, we investigate damages that appear following radiotherapy in two states, single fraction and fractionation irradiation, as an effective sign of cytoskeletal and nuclei structure of vascular wall endothelial cells. Methods: We irradiated human vein endothelial cells (HUVECs) with a Cobalt-۶۰ therapy machine at radiation doses of ۰, ۲, ۴, and ۸ Gy. We stained the skeletal structure of the membrane and nuclei within ۲۴ h after irradiation. This cell line received fractionation radiation therapy at doses from ۰ to ۸ Gy, in sub-fractions of ۲ Gy, after which we stained the cytoskeleton. Morphological parameters such as area and perimeter of the cells and nuclei were determined, and we evaluated the cell shape index (CSI) for cells from each group. Results:  Increasing the irradiation dose from ۰ to ۸ Gy led to a significant decrease of CSI (approximately ۵۶%) and a significant increase of nuclei shape index (approximately ۸۵%; p<۰.۰۵). The cells’ areas and nuclei doubled (p<۰.۰۵). CSI parameter significantly decreased approximately ۲۳% (۴.۵ times lower) during the radiotherapy fraction after application of the ۸ Gy compared with control group cells. Conclusion: After irradiation, we observed broken the membrane filaments that resulted in a new configuration, which led to increased cell and nuclei sizes along with alterations in the cell shape. Radiation therapy led to dose-dependent changes in morphological behavior response of the endothelial cells. Hence, it would be considered as a prognostic factor for behavior of healthy vascular cells in the process of radiotherapy.

نویسندگان

علیرضا محمدکریم

Department of Medical Physics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

منیژه مختاری دیزجی

Department of Medical Physics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

علی کاظمیان

Radiotherapy Oncology Research Center, Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran

هژیر صابری

Department of Radiology, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran

محمد مهدی خانی

Department of Tissue Engineering and Regenerative Medicine, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

سید محمد طباطبایی

Cardiovascular Engineering Lab, Faculty of Biomedical Engineering, Amir kabir University of Technology, Tehran, Iran

محسن بخشنده

Radiology Technology Department, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences and Health Services, Tehran, Iran

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