Effects of Six Weeks of Continuous Training With and Without Nanocurcumin Supplementation on Doxorubicin-induced Hepatotoxicity in an Aging Rat Model

Objective: There are numerous strategies to prevent hepatotoxicity caused by doxorubicin therapy. These strategies include exercise as well as herbal antioxidants such as curcumin to reduce the toxic effects of doxorubicin. This study aims to evaluate the effects of six weeks of continuous training with and without nanocurcumin supplementation on doxorubicin-induced hepatotoxicity in an aging rat model. Methods: We randomly divided ۴۲ Wistar male rats into ۷ groups: control saline, control doxorubicin, nanocurcumin + doxorubicin, nanocurcumin + saline, continuous training + doxorubicin, continuous training + saline, and continuous training + nanocurcumin + doxorubicin. The rats received intraperitoneal injections of D-galactose (۱۰۰ mg/kg) to induce ageing. The training groups ran on a treadmill for six weeks, five days per week with a gradual increase from ۲۵ min/day to ۵۴ min/day at a velocity of ۱۵ m/min to ۲۰ m/min. In the last fifteenth days, rats scheduled to received doxorubicin had a cumulative dose of ۱۵ mg/kg of body weight (daily: ۱ ml/kg). Nanocurcumin supplement (daily: ۱۰۰ mg/kg body weight) was administered to the respective groups. Assessment and analysis were conducted after homogenization of the liver tissue biopsy. Results: Doxorubicin caused a significant decrease in glutathione peroxidase and a slight increase in malondialdehyde in the liver. On the other hand, continuous training with doxorubicin treatment prevented the decrease of glutathione peroxidase and increase in malondialdehyde in the liver that was caused by doxorubicin. Also, six weeks of continuous training with nanocurcumin supplementation caused a significant decrease in malondialdehyde and increased glutathione peroxidase in the liver compared to the control doxorubicin group. Conclusion: Based on the results, the combination of nanocurcumin supplementation and continuous training in the doxorubicin-induced aging rat model have led to a precautionary effect and up-regulation of antioxidant defense. Continuous training appeared to have more beneficial effects than nanocurcumin supplementation in reducing doxorubicin-induced hepatotoxicity.

کلیدواژه ها:

Nanocurcumin ، Training ، Aging ، Hepatotoxicity ، Doxorubicin ، نانوکورکومین ، تمرین تداومی ، سالمندی ، سمیت کبدی ، دوکسوروبیسین