The effects of artemisinin and aqueous and alcoholic extracts of Artemisia annua on Acanthamoeb genotype T۴ in vitro

Background: Free-living amoebae, including acanthamoeba constitute a large group, which live in fresh water, salty and bitter, moist soil, carious plants and some on the stool and has been considered as a medical important agent. The aim of the present study was to determine the effects of aqueous and alcoholic extract of Artemisia annua on trophozoites and cysts of Acanthamoeba in vitro. Materials and Methods: In this experimental research, after genotyping the clinical isolate, the aqueous and alcoholic extracts of Artemisia annua were prepared. Then different concentrations (۱.۲۵, ۲.۵, ۵ and ۱۰ mg/ml) of aqueous and alcoholic extract of plant as well as artemisinin were tested in three different times (۲۴, ۴۸ and ۷۲h) on trophozoites and cysts of Acanthamoeba in vitro. The viability of the parasite was evaluated by trypan blue, MTT and flowcytometry methods.Results: Our result, indicated the anti-acanthamoeba activity of different concentrations of the extract of Artemisia. That, in the presence of ۱۰ mg/ml alcoholic extract in medium culture after ۷۲ hours, ۳۰.۵۱% and ۹۱.۴۰% trophozoite and cyst of Acanthamoeba were found alive. However, in the presence of ۱۰ mg/ml aqueous extract of Artemisia annua, ۵۸.۲۵% and ۸۱.۵۳% trophozoite and cyst respectively were found alive in the in medium culture, after ۷۲ hours.Conclusion: Aqueous and alcoholic extracts of Artemisia annua showed anti-acanthamoebic activity which is dose and time dependent.

کلیدواژه ها:

In vitro ، cyst ، Trophozoite ، Acanthamoeba ، Artemisia annua ، تروفوزوئیت ، کیست ، آکانتاموبا ، عصاره الکلی ، شرایط آزمایشگاهی