A Long Non-coding RNA, PSORS۱C۳, Located Upstream of the Human Oct۴ Gene is Expressed in Pluripotent and Tumor Cell Lines

Objectives: Long non-coding RNAs (lncRNAs), a vast class of recently discovered non-coding genes in the human genome, have been implicated in the regulation of several biological processes, including the maintenance of stem cell pluripotency and neurogenesis. New evidences have emerged that some long IncRNAs act as enhancers for their neighboring genes. Oct۴, also known as POU۵F۱ and Oct۳/۴, functions as a master regulator in maintaining the properties of pluripotency and self-renewal of embryonic stem (ES) cells and embryonal carcinoma (EC) cells. Oct-۴ expression must be tightly regulated; too much or too little expression can lead to cell differentiation. Methods: PSORS۱C۳, an IncRNA, is located upstream of the Oct۴ gene. This IncRNA could potentially impact the level of Oct۴ expression. Here, we have investigated potential expression of PSORS۱C۳ on ۲۳ different human pluripotent and cancer cell lines by means of RT-PCR. Results: Our results revealed a noticeable expression of PSORS۱C۳ both in a well-known pluripotent cell line (NTera۲/NT۲) and five different cancer cell lines (AGS, ۵۶۳۷, Ht-۲۹, HepG۲ and PC۳). Conclusion: We detected the expression of PSORS۱C۳ for the first time in both cancer cell lines and stem cells.

کلیدواژه ها:

PSORS۱C۳ ، IncRNA ، OCT۴ ، OCT۴ ، Stem cell ، PSORS۱C۳ ، lncRNA ، Cancer ، سرطان ، سلول بنیادی