Combined Effects of Curcuma longa and Exercise Training on Kidney and Spleen Tissue Levels of Glutathione Peroxidase and Protein Carbonyl in Rats Exposed to Lead

Objective: Environmental pollution is of major concern today and lead is considered to be one of the most important environmental pollutants. Long-term contact with lead causes harmful effects to humans. This study seeks to determine the effects of Curcuma longa (turmeric extract) consumption and exercise training on glutathione peroxidase and protein carbonyl in kidney and spleen tissues from rats exposed to lead. Methods: We randomly classified ۶۰ male rats into the following six groups of ۱۰ rats per group: ۱) control; ۲) sham (turmeric extract solvent); ۳) lead; ۴) training + lead; ۵) turmeric extract + lead; and ۶) training + lead + turmeric extract. The training program for groups ۳ and ۶ consisted of running on a level treadmill for ۴۰ sessions (eight weeks at five sessions per week) at a speed of ۲۲ to ۱۵ m/min for ۲۶ to ۶۴ minutes. Turmeric extract (۳۰ mg/kg) was injected three times per week for eight weeks. Amounts of glutathione peroxidase and protein carbonyl were measured by ELISA. Results: The amount of protein carbonyl in the kidney and spleen tissues of the lead group increased compared to the sham, training, combined and extract groups. Rats in the combined, extract and practice groups (F=۴.۷۸۷; P=۰.۰۰۲) had lower levels of protein carbonyl in their kidney and spleen tissues compared to the sham group (F=۶.۹۷۰, P=۰.۰۰۰). Glutathione peroxidase levels in the kidney and spleen tissues were less in the lead group compared to the sham group. However these levels in the training, extract, and combined groups increased compared with the sham group (respectively, in kidney and spleen P=۰.۰۵۱, F=۲.۴۶۶ and P=۰/۰۸۶, F=۲.۱۱). Conclusion: Intake of turmeric extract and exercise alone did not cause complete inhibition of the oxidative effects in kidney and spleen tissues. However, exercise and consumption of turmeric extract can be effective in reducing the harmful effects of lead.

کلیدواژه ها:

Exercise training ، Turmeric extract ، Glutathione peroxidase ، Protein carbonyl ، lead ، فعالیت ورزشی ، عصاره زردچوبه ، سرب ، گلوتاتیون پراکسیداز ، پروتئین کربونیل