The Effect of Biosynthesized Zinc Oxide Nanoparticles Capped by Scenedesmus obliquus on Apoptosis and Gene Expression of p۵۳ and VEGF in Breast Cancer Cell Line

Breast cancer is the most prevalent form of cancer in women. This condition poses a significant public health concern that requires further research to understand its prognosis better and proper treatment options. Scenedesmus obiquus algae has unique biological compounds that can be effective in the synthesis of nanoparticles. The objective of this study is to examine how the impact of ZnO NPs, which are produced by the S. obliquus algae, affects cell death and gene expression in a breast cancer cell line. MDA-MB-۲۳۱ cells are a subtype of breast cancer cells known for their high aggressiveness and connection to the triple-negative breast cancer subtype. The impact of biosynthesized ZnO NPs (۰, ۸۰۰, ۱۶۰۰, and ۲۴۰۰ μg/ml) on apoptosis of the MDA-MB-۲۳۱ cell line and VEGF and p۵۳ gene expression in breast cancer cells was assessed. The absorption band at ۳۶۲ nm exhibited an absorption maximum that confirmed the synthesis of ZnO NPs. Zinc oxide nanoparticles exhibited significant toxicity towards MDA-MB-۲۳۱ cell lines, with an IC۵۰ value of ۱۰۵۰ μg/ml. The findings indicated that ۱۶۰۰ μg/ml of ZnO NPs had the most potent inhibitory effect on the growth (۷۱.۱%) of MDA-MB-۲۳۱cultures. The ZnO NPs increased the expression of p۵۳ and reduced the expression of VEGF. This study demonstrates that nanoparticles created using S. obliquus algae, which possess anti-cancer properties, have potential effectiveness in treating cancer. However, it is essential to conduct appropriate in vivo studies to investigate its impact on additional biochemical indicators.

کلیدواژه ها:

Cell death ، green synthesis ، MDA-MB-۲۳۱ cell line ، IC۵۰